A. Title

Analysis of Morphological Structure Differences in Bacteria and Fungi

B. Objectives of the Practical Work

1). For Bacterial Colony Morphology

2). To Study the Morphology of Fungal Colonies (Molds and Yeasts)

C. Tools and Materials

1). Tools

2). Materials

D. Work Procedures

a). Morphology of Bacterial Colonies

b). Morphology of Fungal Colonies

E. Observation Results

A.  Staphylococcus aureus bacteria

 B. Escherichia coli bacteria

C. Mushrooms

F. Discussion Results

     On Tuesday, October 15, 2024, we as representatives of each group conducted a pre-lab to make media for Escherichia Coli and Staphylococcus Aureus bacteria. The materials we used were 150 ml of distilled water, 3 grams of NA, and 1 gram of powder. Previously, the equipment used was sterilized in laminar air flow. Then 150 ml of distilled water, 3 grams of NA, and 1 gram of powder were mixed in a sterilized Erlenmeyer flask. Then heated on a hot plate for 10 minutes at a temperature of 60 degrees. After that, the media made was sterilized in an autoclave at a temperature of 121 degrees for 15 minutes. After the media was sterilized, it was then put in laminar air flow. After that, it was put into the incubator and left for 1 x 24 hours.

      On Wednesday, October 16, we counted Escherichia coli and Staphylococcus Aureus bacteria that we had made on Tuesday, October 12, 2024, which we left for 1x24 hours. Where we got the number of Escherichia Coli bacteria as many as 78, and Staphylococcus Aureus bacteria as many as 645 which were counted using a colony counter tool. The first step taken by the practicum on fungal morphology was to take a sample of fungus on bread using tweezers, which was then placed on a glass slide that had been sterilized using alcohol, after which the sample was dripped with giemsa dye solution, which was then solidified using a cover glass and then observed under a microscope.

According to Syaifuddin (2017) Bread damage is generally caused by the growth of mold, namely Aspergillus Flavus and Rhizopus sp. Some molds can produce aflatoxins which are harmful to humans. One of the mold species that has detrimental properties is Aspergillus Flavus. Aspergillus Flavus mold is the main mold that produces mycotoxins, namely aflatoxins. Aflatoxins are toxins derived from fungi that are known to be deadly and carcinogenic to humans. High aflatoxin content in food can cause poisoning.

According to Mugiono (2015) Yeast is a unicellular fungus that some types are used in making bread or fermentation of alcoholic beverages. Even yeast is used for the manufacture of biological fuel cells, the most commonly used yeast is sacchoromycos cerevisiae which is used for the production of wine, bread, and beer in the form of yeast.

DOCUMENTATION

Observation of Fungal Colony Morphology under a Microscope

Counting the Number of Bacteria Using a Colony Counter

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